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Mnemonics, Simplified Concepts & Thoughts

ABG Sampling (Radial Artery)

Epomedicine, May 22, 2024May 22, 2024

Last updated on May 22, 2024

Start with WIPERS

WIPERS mnemonic for starting patient examination

Then ABG

1. Allen’s test (modified):

  • Exsanguination: Ask the patient to make tight fist for about 30 seconds
  • Occlusion: Occlude the radial and ulnar artery simultaneously with fingers
  • Release:
    • Fist: Hand must stay blanched, else occlusion pressure is not enough (restart the test)
    • Ulnar artery:
      • Reperfusion within 5-15 seconds: Negative Allen’s test (Good blood flow)
      • No reperfusion within 5-15 seconds: Positive Allen’s test (Circulation of ulna artery is not sufficient)

In case of Positive Allen’s test: DO NOT puncture radial artery

2. Bad things:

  • Allergy to LA
  • Absolute contraindications: Cellulitis, Peripheral vascular disease, Arteriovenous fistula, For local anesthesia (Allergy to LA)
  • Relative contraindications: Coagulopathy (including Anticoagulant use)

3. Gather equipment needed

Then Procedure

ABG sampling radial artery
“622.4 – Figure 4 – ABG sampling position – radial artery” by iem-student.org is licensed under CC BY-NC-SA 2.0.

1. Position:

  • Position the patient so that they are sitting or lying down comfortably, ideally with their wrist supported by a pillow.
  • Extend the wrist fully

2. Site preparation:

  • Don the non-sterile gloves
  • Identify site: Palpate the radial artery just proximal to the point of maximum pulsation with non-dominant hand
  • Sterilize the skin with an alcohol wipe and allow it to dry and do not re-palpate

3. Local anesthesia (skin): Offer the patient local anesthesia –

  • 25 G needle (orange) and 2 ml syringe
  • 1% lignocaine

4. ABG sampling:

  • Use 23 G needle (blue) and 2 ml syringe (heparinized with 0.1 ml 1:1000 heparin)
  • Hold the syringe like a pen with a dominant hand
  • Insert the needle at 45-60 degrees with the bevel facing upwards while palpating the artery with other hand
  • Advance the needle slowly until a flash of blood is seen in the needle hub
  • Collect 1-2 ml of arterial blood (bright red and fills quickly in pulsatile fashion)

5. Post sampling:

  • Discard the needle safely
  • Elevate the arm and apply firm pressure to puncture site for 5 minutes
  • Expel any air bubbles from syringe and seal it with a cap
  • Label the syringe (Patient details, FiO2, Body temperature)
  • Roll the syringe in the palms of your hands for 10 seconds (to ensure heparin mixing)
  • Send the sample within 15 minutes of collection for analysis
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Epomedicine. ABG Sampling (Radial Artery) [Internet]. Epomedicine; 2024 May 22 [cited 2025 May 18]. Available from: https://epomedicine.com/clinical-medicine/abg-sampling-radial-artery/.

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