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Biochemistry

USMLE, PGMEE, MBBS, MD/MS

Amylase and Lipase in Acute Pancreatitis

Epomedicine Aug 28, 2019 No Comments BiochemistryGastrointestinal systemInternal medicinePediatrics

Test Rise Peak Return to baseline Lipase 4-6 hours 48 8-14 days Amylase 2-4 hours 24-48 5-7 days Serum lipase elevation has a better diagnostic…

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tyrosinemia
USMLE, PGMEE, MBBS, MD/MS

Tyrosinemia Enzymes : Mnemonic

Epomedicine Aug 12, 2018 No Comments BiochemistryPediatrics

Mnemonic: FAHeem TATtooes because of Histrionic Personality Disorder (HPD) Tyrosine is necessary for biosynthesis of catecholamines, thyroid hormones and melanin pigment. 1. Tyrosinemia type I (Tyrosinosis/Hepatorenal…

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inhibition_Lineweaver_Burk
USMLE, PGMEE, MBBS, MD/MS

Competitive, Non-competitive and Uncompetitive Inhibitors

Epomedicine Aug 10, 2018 1 Comment BiochemistryGeneral conceptsPharmacology

Vmax is the maximum velocity, or how fast the enzyme can go at full ‘‘speed.’’ Vmax is reached when all of the enzyme is in…

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fatty-acid-oxidation
USMLE, PGMEE, MBBS, MD/MS

Beta-oxidation of Fatty Acids : Simplified

Epomedicine Jul 31, 2018 No Comments BiochemistryGeneral concepts

Fatty Acid Activation (Cytoplasm) Before undergoing oxidation, fatty acid must be activated first by conversion into Acyl-CoA by the action of Fatty Acyl-CoA synthase (thiokinase).…

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acetylcoa carboxylase
USMLE, PGMEE, MBBS, MD/MS

Fatty Acid Synthesis : Simplified

Epomedicine Jul 30, 2018 No Comments BiochemistryGeneral concepts

Enzymes: Fatty acid synthase (6 enzymes and 1 Acyl carrier protein molecule) Acetyl-CoA carboxylase (rate-limiting enzyme) Starting material: For palmitate synthesis: Acetyl-CoA For odd number…

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fatty acid
USMLE, PGMEE, MBBS, MD/MS

Structure of Fatty acids and Derivatives : Simplified

Epomedicine Jul 29, 2018 No Comments BiochemistryGeneral concepts

Structure Saturated fatty acid: Single bond (“S” for saturated and single bond) Unsaturated fatty acid: Double bond(s), i.e. “D” for Double bonds, Decreased melting temperature…

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dexamethasone suppression
USMLE, PGMEE, MBBS, MD/MS

Understanding Dexamethasone Suppression Test

Epomedicine Jan 30, 2017 No Comments BiochemistryEndocrine systemInternal medicinePhysiology

The principle behind Dexamethasone Suppression Test (DST) is pretty much simple. It is based on the principle of negative feedback exerted by steroids on pituitary…

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urinary buffers
USMLE, PGMEE, MBBS, MD/MS

Most Important Urinary Buffer: Phosphate or Ammonia?

Epomedicine Jan 27, 2017 2 Comments BiochemistryPhysiologyRenal and Electroloyte

There are multiple choice questions (mcq) which asks: What is the most important urinary buffer? And the choices include both the phosphate and ammonia. Different…

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