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Embryology Week 4: Rule of Fours

Second week is the week of twos. In third week trilaminal germ disc is formed. And now, here I have attempted to fit the fourth week in development of embryo as the week of fours.

Four folds of the embryo

The embryo undergoes:

  1. Lateral folding
  2. Cranio-caudal folding

It forms four folds: 2 lateral, cranial and a caudal fold.

Four subdivision of neural tube

As discussed earlier, by the end of 3rd week – neural plate forms neural folds and neural groove.

Day 22: Middle portion of neural fold closes first at the region of 4th-5th somite

Day 25: Closure of rostral neuropore

Day 28: Closure of caudal neuropore

The closure of the caudal neuropore marks the completion of neurulation and divides neuropore into 4 divisions:

  1. Prosencephalon (Forebrain)
  2. Mesencephalon (Midbrain)
  3. Rhombencephalon (Hindbrain)
  4. Spinal cord

Sulcus limitans divides the neural tube into the dorsal (alar) plate, i.e. sensory and ventral (basal) plate, i.e. motor.

Optical vesicle (future optic nerve, retina, and iris) forms at the basal plate of the prosencephalon.

Neural crest cells: The neuroectodermal cells from lateral part of neural plate detaches and are called neural crest cells. These cells form crests above the neural tube (remember the crest of feathers in the logo of World Health Organization).

Neural crest cells give rise to Parasympathetic nervous system and Ganglia.

Four pharyngeal arches are visible

For more details: Branchial apparatus made easy

Four limb buds begin to grow

Four prominences begin development of face

  1. Stomodeum (primitive mouth) – other prominences grow around the stomodeum
  2. Frontonasal prominence
  3. Maxillary prominences
  4. Mandibular prominences

The embryology of face and falate has already been discussed in: Embryology of face and palate made easy

Four chambered aseptate heart forms and beats

The embryology of heart has already been discussed in: Simplified embryology of heart

Foregut (four-gut) gives respiratory diverticulum

The primitive gut is formed in the 4th week by the incorporation of endoderm of dorsal yolk sac into the developing embryo. It extends from stomodeum to cloaca and consists of 3 parts: foregut, midgut and hindgut.

During the 4th week of gestation of the respiratory diverticulum, appear as a ventral outgrowth of the foregut endoderm.

Four compartmented somites

Somites are mesodermal mass around the neural tube that arises from the paraxial mesoder. It hast 4 compartments:

They begin to be formed at the end of 3rd week and becomes prominent in 4th and 5th week. After day 25, about 3-4 somite pairs grow per day to a total of 42-44 in humas.

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